10108014001

Papain

from Carica papaya

• Synonyme(s) : cystein protease, papain
• Numéro de classification (Commission des enzymes): 3.4.22.2 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: papaya
• Niveau de qualité: 100
• Forme: suspension
• Activité spécifique: 30 U/mg; ~30 units/mg protein (At 25 °C with BAEE as the substrate.)
• Poids mol.: 23 kDa
• Conditionnement: pkg of 10 mL (100 mg)
• Fabricant/nom de marque: Roche
• Concentration: 10-100 mg/mL
• pH optimal: 6.0-7.0
• Conditions d'expédition: wet ice

Description

Description générale

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Application

Papain has been used in the digestion of various brain cells.

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Actions biochimiques/physiologiques

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Caractéristiques et avantages

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Forme physique

Suspension, crystalline, nonsterile

Notes préparatoires

Working concentration: 0.05 to 0.5 mg/ml

Stockage et stabilité

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash