MABT1349

Anti-Desmoglein-2 Antibody, clone CSTEM28

• Synonyme(s) : Cadherin family member 5;HDGC
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: CSTEM28, monoclonal
• Poids mol.: calculated mol wt 122.29 kDa; observed mol wt ~78 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): ELISA: suitable; flow cytometry: suitable; western blot: suitable
• Isotype: IgG2bκ
• Séquence de l'épitope: Extracellular domain
• Numéro d'accès Protein ID: NP_001934
• Numéro d'accès UniProt: Q14126
• Conditions d'expédition: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DSG2(1829)

Description

Description générale

Desmoglein-2 (UniProt: Q14126; also known as Cadherin family member 5, HDGC) is encoded by the DSG2 (also known as CDHF5) gene (Gene ID: 1829) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-2 is a single-pass type I membrane glycoprotein that is found in all tissues and in carcinomas. It serves as a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 50-609), a transmembrane domain (aa 610-634), and a cytoplasmic domain (aa 635-1118). Mutations in DSG2 gene have been linked to familial type of arrhythmogenic right ventricular dysplasia that is characterized y infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias. Desmoglein-2 levels are reported to be consistently upregulated in most cancers where it participates in the maintenance of intracellular junctions that prevents host immune responses. (Ref.: O Brien, CM., et al. (2017). Stem Cells. 35(3); 626-640).

Spécificité

Clone CSTEM28 is a mouse monoclonal antibody that detects Desmoglein-2. It targets an epitope within the extracellular domain.

Immunogène

His-tagged recombinant fragment corresponding to the extracellular domain of human Desmoglein-2.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human Desmoglein-2.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human Desmoglein-2.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Desmoglein-2 in Flow Cytometry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Immunocytochemistry Analysis: A representative lot detected Desmoglein-2 in Immunocytochemistry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

ELISA Analysis: A representative lot detected Desmoglein-2 in ELISA (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Desmoglein-2 , clone CSTEM28, Cat. No. MABT1349, is a mouse monoclonal antibody that detects Desmoglein-2 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1