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MILLIPLEX® Mouse Metabolic Hormone Expanded Panel - Metabolism Multiplex Assay

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.47

Espèces réactives

human

Fabricant/nom de marque

Milliplex®

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex® xMAP®)

Température de stockage

2-8°C

Description générale

Metabolic syndrome is a cluster of conditions that occur together; it includes increased blood pressure, elevated insulin levels, excess body fat around the waist and abnormal cholesterol levels.Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia and central obsesity- all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death.Adults with metabolic syndrome show a low-grade inflammation, whose link with obesity may be dysregulated adipocyte production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes, and obesity related hormones, as well as other cardiovascular disease biomarkers.The MILLIPLEX® Mouse Metabolic Hormone Expansion Panel is a 15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/lysate and culture supernatant samples and mouse serum or plasma: Amylin Active, C-Peptide 2, Ghrelin, GIP, GLP-1 Active or GLP-1 Total, Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, Resistin, Secretin, and TNFα.Note: GLP-1 Active an GLP-1 Total cannot be run together in the same assay.

Spécificité

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

The Mouse Metabolic Hormone Expanded Panel, using the Luminex® xMAP technology, enables the simultaneous analysis of 15 protein biomarkers in mouse serum, plasma and tissue/lysate and culture supernatant samples.There are 16 biomarkers to choose from in this kit. However, this is a 15-plex kit since the following analytes cannot be run simultaneously: GLP-1 (Active) & GLP-1 (Total).The following analytes require the addition of protease inhibitors to the serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor). The protocol recommends serum or plasma samples be run neat. Tissue culture samples may be run neat or may require a dilution with an appropriate control medium. For optimal sensitivity, use an overnight (16 - 18 hour) incubation. Each well requires 10 uL of sample, standard or control.Analytes available:Amylin (Active);C-Peptide 2;Ghrelin (Active);GIP (Total);GLP-1 (Active);GLP-1 (Total);Glucagon;IL-6;Insulin;Leptin;MCP-1/CCL2;Pancreatic Polypeptide (PP);PYY (total);Resistin;Secretin;TNF-α

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Conditionnement

96-well plate

Stockage et stabilité

Recommended storage for kit components is 2 - 8°C.

Informations légales

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Skull and crossbonesEnvironment
GHS06,GHS09

Mention d'avertissement

Danger

Mentions de danger

H302 + H332,H311,H317,H411

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificats d'analyse (COA)

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Contenu apparenté

Comparing xMAP® Sheath Fluid PLUS to Sheath Fluid | MILLIPLEX® Assays

The newer xMAP® Sheath Fluid PLUS formulation contains 3 modes of microbial action to eliminate potential environmental microbial resistance. See how the preparations compare in this performance comparison of the previous xMAP® Sheath Fluid to the new xMAP® Sheath Fluid PLUS formulation in MILLIPLEX® multiplex assays.

Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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