MABE1071

Anti-BAP1 Antibody, clone 1G8

clone 1G8, from mouse

• Synonyme(s) : Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1, Cerebral protein 6
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 1G8, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_004647
• Numéro d'accès UniProt: Q92560
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... BAP1(8314)

Description

Description générale

Ubiquitin carboxyl-terminal hydrolase BAP1 (EC 3.4.19.12; UniProt Q92560; also known as BRCA1-associated protein 1, Cerebral protein 6) is encoded by the BAP1 (also known as KIAA0272, hucep-6) gene (Gene ID 8314) in human. Originally identified and named as a BRCA1-interacting protein, BAP1 is a tumor suppressor that participates in DNA-damage response, DNA synthesis and cell cycle progression. BAP1 belongs to the ubiquitin C-terminal hydrolase (UCH) family of deubiquitin enzymes (DUBs), all members of which, including UCH-L1, UCH-L3 and UCH-L5, are characterized by a conserved UCH catalytic domain (CD; BAP1 a.a. 1-235). BAP1 and UCH-L5 also share a conserved C-terminal ULD domain (a.a. 640-710 of BAP1). The region between BAP1 CD and ULD is important for binding to the HCF-1 protein. In addition, BAP1 contains a C-terminal extension (CTE; a.a. 711-729) following its ULD. Study shows that while CTE tethers BAP1 to the nucleosome core, additional interaction with ASXL1 DEUBiquitinase ADaptor domain (DEUBAD) is needed to allow BAP1 substrate processing by increasing it′s affinity towards ubiquitin. In a similar fashion, UCH-L5 activity is regulated via interaction with DEUBAD of RPN13 and INO80G. BAP1 is implicated in transcription regulation due to its interaction with transcription-related proteins, such as FOXK2, HCF-1, OGT, LSD2, MBD-6, MBD-5, ASXL1 and ASXL2. BAP1 loss leads to a variety of cancers, including metastatic cutaneous and uveal melanoma, pleural mesothelioma, renal cell carcinoma and bladder tumours.

Spécificité

Clone 1G8 targets human BAP1 UCH domain.

Immunogène

Recombinant full-length human BAP1.

Application

Anti-BAP1 Antibody, clone 1G8, Cat. No. MABE1071, is a highly specific mouse monoclonal antibody that targets BAP1 and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Qualité

Evaluated by Western Blotting in transfected HEK293 cells.

Western Blotting Analysis: 2 µg/mL of this antibody detected exogenously expressed human BAP1 in 10 µg lysate from transfected HEK293 cells.

Description de la cible

~80 kDa observed. 80.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1