ABS314
Anti-APPL1 Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat, mouse, human
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... APPL1(26060)
Description générale
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1 (APPL1) is also called DCC-interacting protein 13-alpha (Dip13-alpha). APPL1 is necessary for cell proliferation in response to extracellular signals and links Rab5 to nuclear signal transduction. APPL1 is expressed at high levels in heart, ovary, pancreas, and skeletal muscle tissues.
Immunogène
Recombinant protein corresponding to human APPL1.
Application
Detect Adapter protein containing PH domain, PTB domain & leucine zipper motif 1 using this rabbit polyclonal antibody, Anti-APPL1 Antibody validated for use in western blotting, IP, ICC & IHC.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of L6 and NIH/3T3 cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).
Qualité
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.
Description de la cible
~84 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
APPL1 associates with TrkA and GIPC1 and is required for nerve growth factor-mediated signal transduction.
Lin, Dan C, et al.
Molecular and cellular biology, 26, 8928-8941 (2006)
Kenneth K Y Cheng et al.
Cell metabolism, 9(5), 417-427 (2009-05-07)
Hepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. Here we report that the endosomal adaptor protein APPL1 increases hepatic insulin sensitivity by potentiating insulin-mediated suppression of the gluconeogenic program. Insulin-stimulated activation of Akt and
R Marinho et al.
Journal of cellular physiology, 227(7), 2917-2926 (2011-09-23)
Hepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. The protein kinase Akt plays a central role in the suppression of gluconeogenesis involving forkhead box O1 (Foxo1) and peroxisome proliferator-activated receptor gamma co-activator 1 alpha
Aparajita Lahree et al.
Cell reports, 39(9), 110886-110886 (2022-06-02)
Intracellular pathogens manipulate host cells to survive and thrive. Cellular sensing and signaling pathways are among the key host machineries deregulated to favor infection. In this study, we show that liver-stage Plasmodium parasites compete with the host to sequester a
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