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Key Documents

ABS1030

Sigma-Aldrich

Anti-Apolipoprotein D Antibody

serum, from rabbit

Synonyme(s) :

ApoD, Apolipoprotein D, Apo-D

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

human

Technique(s)

ELISA: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... APOD(347)

Description générale

Apolipoprotein D (ADOP), also known as Apolipoprotein D, Apo-D, ApoD, and encoded by the gene name APOD, is a member of the calycin superfamily and the Lipocalin family. Recent studies have suggested that Apolipoprotein D is involved in the transport and binding of bilin. Additioanlly, studies have demonstrated that Apolipoprotein D is able to transport a variety of ligands in a number of different contexts. Apolipoprotein D is a homodimer located in the plasma, and it also exists as a disulfide-linked heterodimer with APOA2. Apolipoprotein D is expressed in the liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. Apolipoprotein D is primarily localized in HDL (60-65%), with most of the remainder in VHDL and only small amounts in VLDL and LDL.

Immunogène

Protein purified from human plasma corresponding to Apolipoprotein D.

Application

Anti-Apolipoprotein D is an antibody against Apolipoprotein D for use in ELISA, WB.
ELISA Analysis: A representative lot of this antibody detected endogenous Apolipoprotein D in normal human plasma (Knipping, G., et al. Journal of Immunological Methods 202 1997. 85–95)
Western Blotting Analysis: A representative lot of this antibody at 1:5000 dilution detected endogenouse Apolipoprotein D in 5 µl of human plasma (Bottcher, A., et al. J. Lipid Res. 2000. 41: 905–915.)
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Qualité

Evaluated by Western Blotting on purified human Apolipoprotein D

Western Blotting Analysis: A 1:1000 dilution of this antibody detected 0.05 µg of purified human Apolipoprotein D

Description de la cible

~30 kDa observed, additional bands likely due to heavy N-glycosylation on this protein

Forme physique

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Stockage et stabilité

"Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance."

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


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Consulter la Bibliothèque de documents

Preparative free-solution isotachophoresis for separation of human plasma lipoproteins: apolipoprotein and lipid composition of HDL subfractions.
Bottcher, A; Schlosser, J; Kronenberg, F; Dieplinger, H; Knipping, G; Lackner, KJ; Schmitz, G
Journal of Lipid Research null
Characterization of four lipoprotein classes in human cerebrospinal fluid.
Koch, S; Donarski, N; Goetze, K; Kreckel, M; Stuerenburg, HJ; Buhmann, C; Beisiegel, U
Journal of Lipid Research null
G Knipping et al.
Journal of immunological methods, 202(1), 85-95 (1997-03-10)
Apolipoprotein D (apoD), also known as gross cystic disease fluid protein-24 (GCDFP-24), is a minor protein moiety of high-density lipoproteins in human plasma. ApoD is expressed in a subset of breast carcinomas and has been proposed as a tumor marker

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