ABE1317

Anti-phospho Cyclin-dependent kinase 7 (Thr170) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Cyclin-dependent kinase 7, 39 kDa protein kinase, p39 Mo15, CDK-activating kinase 1, Cell division protein kinase 7, Serine/threonine-protein kinase 1, TFIIH basal transcription factor complex kinase subunit
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human, rat
• Technique(s): ChIP: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001790
• Numéro d'accès UniProt: P50613
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pThr170)
• Informations sur le gène: human ... CDK7(1022)

Description

Description générale

Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin protein and they mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Cyclin-dependent kinase 7 (CDK7), also known as Cyclin-dependent kinase 7, or 39 kDa protein kinase (p39-Mo15), CDK-activating kinase 1, Cell division protein kinase 7, Serine/threonine-protein kinase 1, or TFIIH basal transcription factor complex kinase subunit, and encoded by the gene CDK7/CAK/CAK1/CDKN7/MO15/STK1, is a serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CDK7 is inactivated by phosphorylation, thus Cyclin-dependent kinase 7 (Thr107) is an inactive CDK7 protein.

Immunogène

Epitope: Phosphorylated T107

Linear peptide corresponding to human phosphorylated phospho Cyclin-dependent kinase 7 (Thr170).

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-phospho Cyclin-dependent kinase 7 (Thr170) antibody is validated for use in western blotting & ChIP for the detection of phospho Cyclin-dependent kinase 7 (Thr170).

Western Blotting Analysis: A representative lot detected phospho Cyclin-dependent kinase 7 (Thr170) in Cdk7 and Csk1 kinase (Larochelle, S., et al. (2012). Nature Struc. Mole. Biol. 19(11):1108-1115).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho Cyclin-dependent kinase 7 (Thr170) in HCT116 cell lysate treated with doxorubicin (Larochelle, S., et al. (2012). Nature Struc. Mole. Biol. 19(11):1108-1115).

Qualité

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho Cyclin-dependent kinase 7 (Thr170) in 10 µg of A431 cell lysate.

Description de la cible

~ 39 kDa observed

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable