598226

Thiostrepton

≥98% (HPLC), powder, FOXM1 inhibitor, Calbiochem^®

• Synonyme(s) : Thiostrepton, FOXM1 Inhibitor I
• Formule empirique (notation de Hill): C₇₂H₈₅N₁₉O₁₈S₅
• Numéro CAS: 1393-48-2
• Poids moléculaire : 1664.89
• Numéro MDL: MFCD00135828
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• product name: Thiostrepton, Thiostrepton, CAS 1393-48-2, is an antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit.
• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: powder
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: off-white
• Solubilité: DMSO: 10 mg/mL; chloroform: 10 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C72H85N19O18S5/c1-14-26(3)47-63(105)78-30(7)57(99)75-28(5)56(98)76-31(8)58(100)91-72-19-18-40(66-85-43(22-111-66)59(101)77-29(6)55(97)74-27(4)54(73)96)81-52(72)42-21-112-67(83-42)49(34(11)109-69(107)41-20-37(32(9)92)36-16-17-39(79-47)51(95)50(36)80-41)89-60(102)44-24-113-68(86-44)53(71(13,108)35(12)94)90-62(104)45-23-110-65(84-45)38(15-2)82-64(106)48(33(10)93)88-61(103)46-25-114-70(72)87-46/h15-17,20-22,24-26,30-35,39,45,47-49,51-53,79,92-95,108H,4-6,14,18-19,23H2,1-3,7-13H3,(H2,73,96)(H,74,97)(H,75,99)(H,76,98)(H,77,101)(H,78,105)(H,82,106)(H,88,103)(H,89,102)(H,90,104)(H,91,100)/b38-15+
• Clé InChI: NSFFHOGKXHRQEW-DVRIZHICSA-N

Description

Description générale

A thiazole-containing peptide antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit. Inhibits the function of elongation factor G (EF-G) and the dissociation of EF-G from the ribosome. The thiostrepton-resistant gene is also commonly used as a selective marker for recombinant DNA/plasmid technologies.

A thiazole-containing polypeptide antibiotic isolated from Streptomyces azureus. Inhibits bacterial protein synthesis and ribosomal GTPase activity by binding noncovalently, but virtually irreversibly, to the 23S rRNA in the GTPase center of the 50S subunit. Thiostrepton binding directly prevents binding to the ribosome of elongation factor G (EF-G), which also binds specifically to the 23S rRNA. Thiostrepton has proved useful in elucidating the molecular mechanisms of translation, particularly of ribosomal translocation. It is also used for selection, in bacteria of Streptomyces sp., of vectors containing the tsr gene (S. azureus), which encode a 23S rRNA methylase whose action prevents thiostrepton from binding to ribosomes.

Actions biochimiques/physiologiques

Primary Target
EF-G

Avertissement

Toxicity: Standard Handling (A)

Autres remarques

Rodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA96, 9586.
Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res.22, 357.
Saarma, U. and Remme, J. 1992. Nucleic Acids Res.20, 3147.
Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol.58, 895.
Blanco, G., et al. 1992. Gene112, 59.
Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res.19, 1657.
Ryan, P.C., et al. 1991. J. Mol. Biol.221, 1257.
Kutay, U.R., et al. 1990. Biochim. Biophys. Acta1050, 193.
Smokvina, T., et al. 1990. Gene94, 53.
Garrett, R. 1983. Trends Biochem. Sci.8, 189.
Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun.41, 1406.
Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable