596200

Thioflavin T

A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils.

• Synonyme(s) : Thioflavin T, ThT, Thioflavine T, Basic Yellow 1
• Formule empirique (notation de Hill): C₁₇H₁₉ClN₂S
• Numéro CAS: 2390-54-7
• Poids moléculaire : 318.86
• Numéro MDL: MFCD00011944
• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: yellow
• Solubilité: water: 5 mg/mL
• Fluorescence: λ_ex ~385 nm (free); λ_ex ~437 nm; λ_em ~445 nm (free); λ_em ~485 nm
• Conditions d'expédition: ambient
• Température de stockage: 15-25°C
• InChI: 1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1
• Clé InChI: JADVWWSKYZXRGX-UHFFFAOYSA-M

Description

Description générale

A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils. Useful in monitoring stacked β sheet aggregates.

A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils. Useful in monitoring stacked β sheet aggregates. Associates with aggregated fibrils of synthetic β/A4-derived peptides β(1-28) and β(1-40), resulting in a shift in the excitation maximum and an enhanced emission. Excitation max.: ~385 nm (free); ~437 nm (bound); emission max.: ~445 nm (free); ~485 nm (bound).

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
Exhibits enhanced fluorescence upon binding to amyloid fibrils

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Tjernberg, L.O., et al. 1999. J. Biol. Chem.274, 12619.
Naiki, H. and Nakakuki, K. 1996. Lab. Invest.74, 374.
LeVine, H. 1993. Protein Sci.2, 404.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS06,GHS05,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H301,H317,H318,H410
• Conseils de prudence: P261 - P273 - P280 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Sens. 1
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable