5.30650

Luciferase Substrate, CycLuc1

• Synonyme(s) : Luciferase Substrate, CycLuc1, 2-(6,7-dihydro-5h-thiazolo[4,5-f]indol-2-yl)-4,5-dihydrothiazole-4-carboxylicacid
• Formule empirique (notation de Hill): C₁₃H₁₁N₃O₂S₂
• Poids moléculaire : 305.38
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Pureté: ≥94% (HPLC)
• Niveau de qualité: 100
• Forme: powder
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: yellow
• Solubilité: DMSO: 100 mg/mL
• Température de stockage: 2-8°C

Description

Description générale

A synthetic cyclic alkylamino luciferin that exhibitsproperties that are superior to those of D-luciferin. Binds to luciferase with higheraffinity and emits about 5.7-fold more light than aminoluciferin and3.2-fold more light than D-luciferin. Its superior light output is maintainedover a wide range (1-100 µM). Shown to readily cross the blood-brain barrier inmice and provides much improved noninvasive bioluminescence imaging (BLI) ofthe brain at a lower dose when compared to D-luciferin. Yields about 10-foldhigher bioluminescent signal than D-luciferin when Luc2 luciferase expressing4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice.A rapid (4-5 min), long-lasting, and steady signal peak is observed followingan i.v injection of CycLuc1 in mice.

Application

Luciferase Substrate, CycLuc1 has been used for transfecting HEK293 cells to study the inhibition of intracellular luciferase crystallization by D-luciferin and its synthetic analogues.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
Luciferase

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Autres remarques

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable