234567M

Concanavalin A, Canavalia ensiformis

Concanavalin A, Canavalia ensiformis, CAS 11028-71-0, is a carbohydrate-binding protein that agglutinates various animal cells. Restores the growth pattern of tumor cells to that of normal cells.

• Synonyme(s) : Concanavalin A, Canavalia ensiformis, Con A, Jack Bean Hemagglutinin
• Numéro CAS: 11028-71-0
• Numéro MDL: MFCD00071069
• Code UNSPSC : 12352202

Propriétés

• Niveau de qualité: 100
• Forme: lyophilized
• Puissance: ≤20 μg/mL lectin agglutination activity (as determined from serial dilutions of a 1 mg/mL solution)
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• pl: 4.5 - 5.5
• Température de stockage: 2-8°C

Description

Description générale

Carbohydrate-binding protein that agglutinates various animal cells. Also known to restore the growth pattern of tumor cells to that of normal cells. T lymphocyte activator. Has binding sites for metal ions, α-D-mannose, and α-D-glucose.

Carbohydrate-binding protein that agglutinates various animal cells. Also known to restore the growth pattern of tumor cells to that of normal cells. T lymphocyte activator. Has binding sites for metal ions, α-D-mannose, and α-D-glucose. Has a pI of 4.5-5.5.

Avertissement

Toxicity: Harmful (C)

Notes préparatoires

To dissolve, add Con A slowly to a solution of 10 mM HEPES buffered saline, 100 µM CaCl₂, pH 8.5. Allow the lectin to dissolve without agitation.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) or add 0.08% NaN₃ and refrigerate (4°C). Stock solutions are stable for up to 6 months at -20°C or for up to 3 months at 4°C.

Remarque sur l'analyse

The agglutination activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25°C; requires type A human blood.

Autres remarques

Gantner, F., et al. 1995. Hepatology 21, 190.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317 - H334 - H361
• Conseils de prudence: P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Classification des risques: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable