17-10499
Magna MeRIP m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine
Synonyme(s) :
Magna MeRIP m6A Kit, m6A Profiling Kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41105331
Nomenclature NACRES :
NA.84
Produits recommandés
Niveau de qualité
Description générale
N6-methyladenosine (m6A) represents an abundant RNA modification that is conserved across many different species, ranging from plants, to yeast, to mammals. Similar to the 5-methylcytosine (5-mc) modification of DNA, m6A is a reversible chemical modification of RNA. m6A RNA modifications are an emerging topic in controlling cell fate transitions in mammalian embryonic stem cells. m6A is one of the most prevalent modifications on both mRNAs and noncoding RNAs in eukaryotes with an estimated 12,000 m6A sites in over 7,000 genes. The mark is deposited by a heterodimer of methyltransferase-like 3 and 14 (Mettl3 and Mettl14) and can be removed by the RNA demethylase enzymes FTO and ALKBH5. Obesity risk gene FTO encodes the first identified m6A demethylase. Mutations in FTO have been associated with increased risk for obesity and type II diabetes. Recently, impairment of the status of m6A regulation has lead to prolonged expression of Nanog in ES cells and the inability to exit self-renewal stages toward differentiation.
Methylated (m6A) RNA immunoprecipitation (MeRIP) is a method to monitor the status of m6A and map the location of m6A RNA modifications transcriptome-wide. The Magna MeRIP M6A Kit uses the MeRIP method to enable identification and transcriptome-wide profiling of m6A RNA modifications. In the MeRIP assay, RNA is chemically fragmented into 100 nucleotides or smaller fragments followed by magnetic immunoprecipitation with a monoclonal antibody toward m6A. After immunoprecipitation, isolated RNA fragments can be subjected with qRT-PCR or RNA sequencing (MeRIP-seq). The streamlined protocol is easy to process and can produce results with high SN ratios.
Methylated (m6A) RNA immunoprecipitation (MeRIP) is a method to monitor the status of m6A and map the location of m6A RNA modifications transcriptome-wide. The Magna MeRIP M6A Kit uses the MeRIP method to enable identification and transcriptome-wide profiling of m6A RNA modifications. In the MeRIP assay, RNA is chemically fragmented into 100 nucleotides or smaller fragments followed by magnetic immunoprecipitation with a monoclonal antibody toward m6A. After immunoprecipitation, isolated RNA fragments can be subjected with qRT-PCR or RNA sequencing (MeRIP-seq). The streamlined protocol is easy to process and can produce results with high SN ratios.
Application
The Magna MeRIP m6A Kit streamlines the MeRIP (Methylated RNA Immunoprecipitation) method to enable identification and transcriptome-wide profiling of m6A (N6-methyladenosine) RNA methylation sites. Suitable for MeRIP-Seq and qRT-PCR of methylated RNA transcripts.
Composants
RNA Fragmentation Buffer 10X (0.5 mL);0.5M EDTA (0.5 mL);IP Buffer 5X (15 mL);Magnetic Beads A/G Blend (315 μL);Anti-N6-methyladenosine (m6A), clone 17-3-4-1 (100 μg);RNase Inhibitor (90 μL);N6-Methyladenosine, 5′-monophosphate sodium salt (m6A) (10 mg);Normal Mouse IgG (125 μg);MeRIP Primers Human EEF1A1 Positive (75 μL);MeRIP Primers Human EEF1A1 Negative (75 μL)
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique