07-1016
Anti-phospho-Upf1 (Ser1127) Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
Regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, human
Réactivité de l'espèce (prédite par homologie)
chicken (based on 100% sequence homology), zebrafish (based on 100% sequence homology), yeast (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pSer1127)
Informations sur le gène
human ... UPF1(5976)
Description générale
Regulator of nonsense transcripts 1 (UniProt: Q92900; also known as ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1) is encoded by the UPF1 (also known as KIAA0221, RENT1) gene (Gene ID: 5976) in human. Upf1 is a member of the DNA2/NAM7 helicase family. It is a RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. It is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation. It is phosphorylated by SMG1 and phosphorylation is considered as an essential step in NMD and is required for formation of mRNA surveillance complexes. Phosphorylated Upf1 is recognized by EST1B/SMG5, SMG6, and SMG7, which provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways. A hyper-phosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. The ATPase activity of Upf1 is required for disassembly of mRNPs undergoing NMD and is also essential for embryonic viability. Two isoforms of Upf1 have been reported that are produced by alternative splicing. Upf1 contains a UPF1-type zinc-finger domain (aa 121-272) and a nucleotide-binding domain (aa 506-510).
Spécificité
This rabbit polyclonal antibody detects Regulator of nonsense transcripts 1 (RENT1) in multiple species. It targets an epitope within 9 amino acids surrounding phosphorylated Ser1127.
Immunogène
Epitope: Phosphorylated Ser1127
KLH-conjugated linear peptide corresponding to Upf1 phosphorylated at Ser1127.
Application
Anti-phospho-Upf1 (Ser1127), Cat. No. 07-1016, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 1 (RENT1) phosphorylated at Ser1127 and has been tested in Immunoprecipitation, peptide Inhibition Assay, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-Upf1 (Ser1127) in 10 µg of A431 treated with Calyculin A and Okadaic Acid.
Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-Upf1 (Ser1127) in NIH3T3 treated with Calyculin A and Okadaic Acid.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho-Upf1 (Ser1127) in NIH3T3 treated with Calyculin A and Okadaic Acid.
Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-Upf1 (Ser1127) in NIH3T3 treated with Calyculin A and Okadaic Acid.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho-Upf1 (Ser1127) in NIH3T3 treated with Calyculin A and Okadaic Acid.
Qualité
Evaluated by Western Blotting in NIH3T3 treated with Calyculin A and Okadaic Acid.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Upf1 (Ser1127) in 10 µg lysate from NIH3T3 treated with Calyculin A (50 nM) and Okadaic Acid (500 nM) for 30 minutes.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Upf1 (Ser1127) in 10 µg lysate from NIH3T3 treated with Calyculin A (50 nM) and Okadaic Acid (500 nM) for 30 minutes.
Description de la cible
~140 kDa observed. 125 kDa calculated.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Yang Zhao et al.
Nature communications, 11(1), 3345-3345 (2020-07-06)
Nonsense-mediated mRNA decay (NMD) is an evolutionarily conserved RNA decay mechanism that has emerged as a potent cell-intrinsic restriction mechanism of retroviruses and positive-strand RNA viruses. However, whether NMD is capable of restricting DNA viruses is not known. The DNA
Feng Wang et al.
Nature communications, 14(1), 4760-4760 (2023-08-09)
Long-read RNA sequencing (RNA-seq) is a powerful technology for transcriptome analysis, but the relatively low throughput of current long-read sequencing platforms limits transcript coverage. One strategy for overcoming this bottleneck is targeted long-read RNA-seq for preselected gene panels. We present
Tatsuaki Kurosaki et al.
Genome biology, 22(1), 317-317 (2021-11-18)
Fragile X syndrome (FXS) is an intellectual disability attributable to loss of fragile X protein (FMRP). We previously demonstrated that FMRP binds mRNAs targeted for nonsense-mediated mRNA decay (NMD) and that FMRP loss results in hyperactivated NMD and inhibition of
Hanae Sato et al.
Nature communications, 12(1), 7203-7203 (2021-12-12)
Nonsense-mediated mRNA decay (NMD) is an mRNA degradation pathway that eliminates transcripts containing premature termination codons (PTCs). Half-lives of the mRNAs containing PTCs demonstrate that a small percent escape surveillance and do not degrade. It is not known whether this
Sébastien Durand et al.
Nature communications, 7, 12434-12434 (2016-08-12)
Many gene expression factors contain repetitive phosphorylation sites for single kinases, but the functional significance is poorly understood. Here we present evidence for hyperphosphorylation as a mechanism allowing UPF1, the central factor in nonsense-mediated decay (NMD), to increasingly attract downstream
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