06-284-AF488

Anti-Nitrotyrosine Antibody Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• Synonyme(s) : Nitrotyrosine AlexaFluor^™ 488 Conjugate
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): all
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: nitration (Tyr)
• Informations sur le gène: human ... NOS1(4842)

Description

Description générale

Nitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration. In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.

Spécificité

This rabbit polyclonal antibody detects tyrosine nitrated proteins.

Immunogène

Epitope: Nitrated Tyrosine

Nitrated Keyhole Limpet hemocyanain (KLH).

Application

Anti-Nitrotyrosine AlexaFluor 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.

Research Category
Signaling

Research Sub Category
Developmental Signaling

The unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.

Qualité

Evaluated by Immunocytochemistry in A431 cells treated with peroxytnitrite.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.

Forme physique

Affinity purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable