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Key Documents

04-135

Sigma-Aldrich

Anti-Brachyury Antibody, clone 3E4.2

clone 3E4.2, Upstate®, from mouse

Synonyme(s) :

T brachyury (mouse) homolog, T brachyury-like, T protein, T, brachyury homolog (mouse), transcription factor T

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

3E4.2, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Upstate®

Technique(s)

western blot: suitable

Entrée

sample type mesenchymal stem cell(s)

Isotype

IgG1κ

Numéro d'accès NCBI

NP_003172

Numéro d'accès UniProt

O15178

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TBXT(6862)

Description générale

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Spécificité

Reactivity with other species has not been tested.
This monoclonal antibody recognizes the brachyury protein.

Immunogène

Epitope: C-terminus
Synthetic human brachyury peptide, a.a. 224-435.

Application

Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.

Qualité

Routinely evaluated by Western blot on NT2 cell lysates.

Western Blotting:
Recommended working dilutions are 1:1000.

Description de la cible

47 kDa

Forme physique

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Brachyury, a crucial regulator of notochordal development, is a novel biomarker for chordomas.
Vujovic, S, et al.
The Journal of Pathology, 209, 157-165 (2006)
Chromatin remodeling during mouse and human embryonic stem cell differentiation.
Golob, Jonathan L, et al.
Developmental Dynamics, 237, 1389-1398 (2008)
T P Yamaguchi et al.
Genes & development, 13(24), 3185-3190 (2000-01-05)
Wnt3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is required for paraxial mesoderm development. In its absence cells adopt ectopic neural fates. Embryos lacking the T-box-containing transcription factors, Brachyury or Tbx6
Development of antibodies to human embryonic stem cell antigens.
Cai, Jingli, et al.
BMC Developmental Biology, 5, 26-26 (2005)
Hematopoiesis from human embryonic stem cells: overcoming the immune barrier in stem cell therapies.
Priddle, Helen, et al.
Stem Cells, 24, 815-824 (2006)

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