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Key Documents

TRUEDEXS

Sigma-Aldrich

TrueGel3D Polymer

Modified for slow gelling (SLO-DEXTRAN), allow cell recovery

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

shipped in

dry ice

storage temp.

−70°C

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General description

Dextrose polymers are functionalized with thiol-reactive groups, which react at a slower rate with thiol groups of crosslinkers (CD/PEG crosslinkers) to form stable hydrogels. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of homogenous gel formation. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

SLO-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

Features and Benefits

  • Contains dextrose polymer functionalized with thiol reactive groups for slow gelation
  • Degradable polymer

Components

TRUEDEXS contains SLO-DEXTRAN solution in phosphate buffer, TrueGel3D thioglycerol and water

Caution

Avoid frequent freeze-thaw cycles and store SLO-DEXTRAN in small portions

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

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Articles

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Related Content

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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