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P8483

Sigma-Aldrich

Polyamine Supplement (1000×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Polyamine cell culture tested

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About This Item

UNSPSC Code:
12352205
NACRES:
NA.75

biological source

non-animal source

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

General description

Polyamine Supplement (1000×) is a general medium supplement specially formulated to boost cell growth for enhanced viral and protein expression. One mL supplement is recommended for 1 liter of medium.

Application

Polyamine Supplement (1000×) has been used with serum-free medium (SFM) to culture human lymphocytes from peripheral blood mononuclear cells (PBMCs). It has also been used in combination with antioxidant supplement (A 1345) to culture zygotes.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Min Kyoung Jeon et al.
BMC biotechnology, 10, 70-70 (2010-09-22)
Serum-containing medium (SCM), which has a number of poorly defined components with varying concentrations, hampers standardization of lymphocyte cultures. In order to develop a serum-free medium (SFM) for the expansion of human lymphocytes from peripheral blood mononuclear cells (PBMCs), a
Yu Chen et al.
Nature methods, 18(5), 528-541 (2021-05-05)
Human pluripotent stem cells (hPSCs) are capable of extensive self-renewal yet remain highly sensitive to environmental perturbations in vitro, posing challenges to their therapeutic use. There is an urgent need to advance strategies that ensure safe and robust long-term growth
Yanrong Zhou et al.
Veterinary microbiology, 250, 108839-108839 (2020-10-02)
Like obligate intracellular parasites, viruses co-opt host cell resources to establish productive infections. Polyamines are key aliphatic molecules that perform important roles in cellular growth and proliferation. They are also needed for the successful multiplication of various viruses. Little is

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