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Key Documents

M7150

Sigma-Aldrich

Murashige and Skoog Vitamin Powder

1000 ×, powder, suitable for plant cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

concentration

1000 ×

technique(s)

cell culture | plant: suitable

pH

3.5-4.5 (1.031 g/10 mL)

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Vitamin powder (M7150) can be combined with Murashige and Skoog micro/macro-nutrients to generate basal media which when supplemented with organics, agar, auxins and cytokinins yield complete medium for growth plant tissue culture.

Reconstitution

Use at a concentration of one mL per liter of prepared medium to achieve the proper final concentration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elsa Matthus et al.
Plant physiology, 179(4), 1754-1767 (2019-01-31)
Phosphate (Pi) deficiency strongly limits plant growth, and plant roots foraging the soil for nutrients need to adapt to optimize Pi uptake. Ca2+ is known to signal in root development and adaptation but has to be tightly controlled, as it
Saxena et al.
Plant science : an international journal of experimental plant biology, 155(2), 133-140 (2000-05-18)
Efficient protocols have been established for both direct and indirect regeneration of plants in Pelargonium graveolens Indian cultivar Hemanti (Algerian type). Murashige and Skoog's (MS) medium [T. Murashige, F. Skoog, A revised medium for rapid growth and bioassays with tobacco

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