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G5768

Sigma-Aldrich

Gamborg′s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

Synonym(s):

B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

fine powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Related Categories

General description

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Application

Gamborg′s B-5 Basal Salt Mixture has been used:
  • as a constituent during the preparation of TEX buffer
  • as a media to maintain potato suspension and callus cultures
  • as a media in the primary-node transformation method

Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Formula variant

With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 3.1 grams of powder per liter of medium.

Pictograms

Flame over circle
GHS03

Signal Word

Warning

Hazard Statements

H272

Hazard Classifications

Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Ashish Baldi et al.
Biotechnology letters, 30(9), 1671-1677 (2008-04-23)
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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