GERPN2235
ECL™ Select Western Blotting Detection Reagent
Cytiva RPN2235
Synonym(s):
chemiluminescent detection reagent, Western blotting detection reagent
About This Item
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2235
Related Categories
General description
Amersham™ ECL™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham™ ECL™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.
Amersham™ ECL™ Select typically exhibits a dynamic range that is complementary to Amersham™ ECL™ Prime, and similar to Amersham™ ECL™ Advance.
Application
Features and Benefits
- Improved sensitivity: Produces a very high signal intensity
- Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins
- Works with a wide range of antibody dilutions, reducing the need for lengthy optimization
- Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham™ Hyperfilm
- Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
- Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond® ECL™ (nitrocellulose) membranes
Components
Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane
complete packsize
for 1000 cm2 membrane
Analysis Note
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Troubleshooting of Detection Methods of GST-tagged Proteins
Protocols
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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