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GERPN2106

ECL™ Western Blotting Reagents

Cytiva RPN2106

Synonym(s):

ECL detection kit

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About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

form

liquid

feature

wetted part: no

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2106

color

Detection reagent 1: Colorless Detection reagent 2: Colorless

solubility

water: soluble

storage temp.

2-8°C

General description

Amersham™ ECL™ Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL™ Western Blotting Detection Reagents in combination with Hybond® ECL™ membranes form a complete solution for Western blot applications.

Emission duration max. 2 hours

Contents include the following:
GERPN2109, 62.5 mL each of ECL™ Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2).

Application

Western Blotting

Features and Benefits

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL™ Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL™ or with CCD imager
  • Recommended membrane Hybond ECL™

Components

Sufficient for detection of 4000 cm2 of membrane:
250 mL Detection reagent 1(color : colorless)
250 mL Detection reagent 2 (color: colorless)

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Linhua Wang et al.
Journal of cellular physiology, 236(6), 4625-4639 (2021-01-17)
Sepsis-induced myocardial dysfunction (SIMD), a deadly symptom in sepsis patients, is mainly caused by cardiovascular inflammation. However, it remains unclear how systemic inflammation triggers and aggravates cardiovascular inflammation in the pathogenesis of SIMD. This study found that proinflammatory cytokines and

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Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service