GE17-0981-01
CNBr Activated Sepharose™ 4 Fast Flow
Cytiva 17-0981-01, pack of 10 g
Synonym(s):
Affinity chromatography resin
About This Item
Recommended Products
ligand
cyanogen bromide
packaging
pack of 10 g
manufacturer/tradename
Cytiva 17-0981-01
storage condition
(Unsused: 2 - 8°C - Used: 2 - 8°C in 20% Ethanol)
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-11(Ligand dependent)
working range
3-11(ligand dependent)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
CNBr-activated Sepharose™ 4 Fast Flow is based on the well established Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. CNBr-activated Speharose 4 Fast Flow is designed for multipoint attachment of protein ligands containing amino groups.
The preparation and use of affinity chromatography media by coupling biospecific ligands to CNBr-activated Sepharose™ 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure.
CNBr-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, CNBr-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- CNBr-activated BioProcess medium designed for coupling of large amino-containing ligands.
- Easy, rapid and efficient coupling
- This resin allows for multi-point attachment of protein ligands which minimizes ligand leakage
- BioProcess medium supported for industrial applications and well-established in approved processes
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Certificates of Analysis (COA)
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Protocols
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Selectivity of HIC medium from GE Healthcare is based on properties such as hydrophobicity, porosity and particle size.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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