C9142

Cyanogen bromide-activated-Sepharose^™ 4B

lyophilized powder

• Synonym(s): Cyanogen bromide-activated Agarose
• CAS Number: 68987-32-6
• MDL number: MFCD00163385
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• form: lyophilized powder
• technique(s): affinity chromatography: suitable
• matrix: Sepharose 4B
• matrix active group: cyanate or related structures
• matrix spacer: 1 atom (when ligands are coupled via isourea derivative or related linkage)
• swelling: 1 g swells to 4-5 mL
• particle size: 45—165 μm
• storage temp.: 2-8°C

Description

General description

Cyanogen bromide-activated-Sepharose^™ is suitable for use in affinity chromatography and protein chromatography. It has been used to discover insecticidal agents, raise polyclonal antibodies against yeast invertase glycosyls, and study immunological processes of xenograft rejection.

C9142-15G′s updated product number is GE17-0430-01

Application

Cyanogen bromide-activated-Sepharose^™ 4B has been used:

• in the purification of clusterin from serum by an immunoaffinity-based separation procedure
• in affinity chromatography to isolate Leishmania donovani protein antigens from the urine of visceral leishmaniasis patients
• in affinity chromatography for the isolation of haptoglobin from human serum

Physical form

Lyophilized powder stabilized with lactose and dextran

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves