D5523

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): DME, DMEM
• MDL number: MFCD00217342
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: L-glutamine: yes; NaHCO₃: no; sodium pyruvate: yes; phenol red: yes; HEPES: no; glucose: low
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).

Quantity

Formulated to contain 10.0 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319
• Precautionary Statements: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable