D5523
Dulbecco′s Modified Eagle′s Medium - low glucose
With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME, DMEM
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Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low
shipped in
ambient
storage temp.
2-8°C
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General description
Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.
Application
Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).
Quantity
Formulated to contain 10.0 grams of powder per liter of medium.
Reconstitution
Supplement with 3.7 g/L sodium bicarbonate.
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supplement
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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