D2429
Dulbecco′s Modified Eagle′s Medium - low glucose
10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
DME, DMEM
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About This Item
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Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
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General description
Dulbecco′s Modified Eagle′s Medium (DME) is a modification of basal medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose.
Application
Dulbecco′s Modified Eagle′s Medium-low glucose has been used:
- during organoid embedding for neutralizing thawed collagen I
- to culture melanoma cancer cell lines
- to store the cancer tissue samples
- as a differentiation culture medium for C2C12 cells
Reconstitution
Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.
also commonly purchased with this product
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Description
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supplement
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Maria Guix et al.
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Novel approaches to develop naturally-induced drug delivery in tumor environments in a deterministic and controlled manner have become of growing interest in recent years. Different polymeric-based microstructures and other biocompatible substances have been studied taking advantage of lactic acidosis phenomena
Yi Li et al.
Physiological genomics, 50(3), 158-168 (2018-01-18)
Folic acid supplements taken during pregnancy can prevent neural tube defects and other developmental abnormalities. Here, we explored the effects of folate supplementation on gene expression and DNA methylation during C2C12 differentiation. Based on the folic acid concentration, this study
Tejas Shah et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 84, 526-534 (2016-10-04)
Folic acid (FA) and iron are essential supplements during pregnancy. Similarly effects of vitamin B12 (B12) inadequacy and high folate and low B12 status, on pregnancy outcome are available. However there are no mandatory recommendations for B12. There are many
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Folate, an essential micronutrient, is a critical cofactor in one-carbon metabolism for many cellular pathways including DNA synthesis, metabolism and maintenance. Folate deficiency has been associated with an increased risk of neurological disease, cancer and cognitive dysfunction. Dihydrofolate reductase (DHFR)
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