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B7554

Sigma-Aldrich

Anti-c-Myc−Biotin antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

Synonym(s):

Anti-c-Myc, Monoclonal Anti-c-Myc−Biotin antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

9E10, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

0.8 mg/mL

technique(s)

western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein

UniProt accession no.

P01106

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)

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General description

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Specificity

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Immunogen

synthetic peptide of the human p62c-Myc protein.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
The antibody is suitable for immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Steven R Bates et al.
Applied physics letters, 95(7), 73705-73705 (2009-09-25)
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association
Illari Salvatori et al.
Journal of neurochemistry, 146(5), 585-597 (2018-05-21)
TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein and a major component of protein aggregates found in amyotrophic lateral sclerosis and several other neurodegenerative diseases. TDP-43 exists as a full-length protein and as two shorter forms of 25 and
Ilja Demuth et al.
Oncogene, 23(53), 8611-8618 (2004-10-07)
DNA interstrand crosslinks (ICLs) are critical lesions for the mammalian cell since they affect both DNA strands and block transcription and replication. The repair of ICLs in the mammalian cell involves components of different repair pathways such as nucleotide-excision repair
Linglin Xie et al.
Biochimica et biophysica acta, 1782(2), 99-108 (2008-01-09)
Adiponectin and leptin are two adipokines secreted by white adipose tissue that regulate insulin sensitivity. Previously we reported that adiponectin but not leptin release depends on GGA-coated vesicle formation, suggesting that leptin and adiponectin may follow different secretory routes. Here
Sarah Tulin et al.
BMC developmental biology, 10, 83-83 (2010-08-07)
As important regulators of developmental and adult processes in metazoans, Fibroblast Growth Factor (FGF) proteins are potent signaling molecules whose activities must be tightly regulated. FGFs are known to play diverse roles in many processes, including mesoderm induction, branching morphogenesis
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