G6639
Guanidine thiocyanate
≥97% (titration)
Synonym(s):
Guanidinium rhodanide, Guanidinium thiocyanate
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About This Item
Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
Molecular Weight:
118.16
Beilstein:
3563461
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.55
Recommended Products
biological source
synthetic
Quality Level
Assay
≥97% (titration)
form
powder or crystals
technique(s)
RNA extraction: suitable
color
white to off-white
mp
120-122 °C (lit.)
solubility
water: 4 M, clear, colorless
anion traces
chloride (Cl-): ≤2.5%
SMILES string
SC#N.NC(N)=N
InChI
1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChI key
ZJYYHGLJYGJLLN-UHFFFAOYSA-N
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Application
Guanidine thiocyanate has been used:
- as a denaturing agent to study its effects on surface activity of globular protein
- for the extraction of total RNA from primary hepatocytes
- as a component of the incubating buffer for extraction of DNA from human bone
- as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
- as a component of lysis solution for extraction of RNA from rat pancreatic tissues
Chaotropic agent and strong denaturant; solubilizes cells.
Biochem/physiol Actions
Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Supplementary Hazards
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Emilia Bramanti et al.
Analytical biochemistry, 351(1), 100-113 (2006-01-28)
In this article, a multidimensional dynamic surface tension detector (DSTD), in a parallel configuration with a UV-visible diode array absorbance detector, is presented in a novel flow injection analysis (FIA) application to study the effects of chemical denaturants urea, guanidinium
The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates.
Julian R Peat et al.
F1000Research, 6, 526-526 (2017-06-06)
Methylation of CG dinucleotides constitutes a critical system of epigenetic memory in bony vertebrates, where it modulates gene expression and suppresses transposon activity. The genomes of studied vertebrates are pervasively hypermethylated, with the exception of regulatory elements such as transcription
Nicolas Chuvin et al.
Cellular and molecular gastroenterology and hepatology, 4(2), 263-282 (2017-07-29)
Transforming growth factor beta (TGFβ) acts either as a tumor suppressor or as an oncogene, depending on the cellular context and time of activation. TGFβ activates the canonical SMAD pathway through its interaction with the serine/threonine kinase type I and II
Kai Y Mak et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(9), 1434-1443 (2015-05-23)
Angiotensin converting enzyme 2 (ACE2) which breaks down profibrotic peptide angiotensin II to antifibrotic peptide angiotensin-(1-7) is a potential therapeutic target in liver fibrosis. We therefore investigated the long-term therapeutic effect of recombinant ACE2 using a liver-specific adeno-associated viral genome
Yuji Suzuki et al.
Bioscience, biotechnology, and biochemistry, 72(7), 1951-1953 (2008-07-08)
High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds
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