ABS314
Anti-APPL1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... APPL1(26060)
General description
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1 (APPL1) is also called DCC-interacting protein 13-alpha (Dip13-alpha). APPL1 is necessary for cell proliferation in response to extracellular signals and links Rab5 to nuclear signal transduction. APPL1 is expressed at high levels in heart, ovary, pancreas, and skeletal muscle tissues.
Immunogen
Recombinant protein corresponding to human APPL1.
Application
Detect Adapter protein containing PH domain, PTB domain & leucine zipper motif 1 using this rabbit polyclonal antibody, Anti-APPL1 Antibody validated for use in western blotting, IP, ICC & IHC.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of L6 and NIH/3T3 cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.
Target description
~84 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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APPL1 associates with TrkA and GIPC1 and is required for nerve growth factor-mediated signal transduction.
Lin, Dan C, et al.
Molecular and cellular biology, 26, 8928-8941 (2006)
Kenneth K Y Cheng et al.
Cell metabolism, 9(5), 417-427 (2009-05-07)
Hepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. Here we report that the endosomal adaptor protein APPL1 increases hepatic insulin sensitivity by potentiating insulin-mediated suppression of the gluconeogenic program. Insulin-stimulated activation of Akt and
R Marinho et al.
Journal of cellular physiology, 227(7), 2917-2926 (2011-09-23)
Hepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. The protein kinase Akt plays a central role in the suppression of gluconeogenesis involving forkhead box O1 (Foxo1) and peroxisome proliferator-activated receptor gamma co-activator 1 alpha
Aparajita Lahree et al.
Cell reports, 39(9), 110886-110886 (2022-06-02)
Intracellular pathogens manipulate host cells to survive and thrive. Cellular sensing and signaling pathways are among the key host machineries deregulated to favor infection. In this study, we show that liver-stage Plasmodium parasites compete with the host to sequester a
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