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Key Documents

AB153-I

Sigma-Aldrich

Anti-Fluorescent Gold Antibody

from rabbit

Synonym(s):

Fluorescent Marker

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity (predicted by homology)

all

technique(s)

immunohistochemistry: suitable (paraffin)

shipped in

wet ice

target post-translational modification

unmodified

General description

Fluorescent Gold (hydroxystilbamidine), better known as Fluorogold, has been a very popular fluorescent marker used to retrogradely label projection neurons. AB153 also reacts with aminostilbamidine quite nicely in frozen, 4% PFA fixed tissues.

Specificity

This antibody will react with any species sample treated with Fluorescent Gold.

Immunogen

KLH-conjugated Fluorescent Gold.

Application

Anti-Fluorescent Gold Antibody is a highly specific rabbit polyclonal antibody, that targets Fluoro-Gold & has been tested in IHC (Paraffin).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Fluorescent Gold treated neurons in rat frontal cortex tissues (Chang, H. T., et al. (1990). J Neurosci Methods. 35(1):31-37.).

Quality

Evaluated by Immunohistochemistry in fixed frozen Fluorescent Gold treated rat cerebral cortex tissues.

Immunohistochemistry Analysis: A 1:10,000 dilution of this antibody detected Fluorescent Gold in fixed frozen Fluorescent Gold treated rat cerebral cortex tissues.

Physical form

Format: Purified

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sofia Meyer Zu Reckendorf et al.
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Nature neuroscience, 26(1), 116-130 (2022-12-23)
Corticothalamic pathways, responsible for the top-down control of the thalamus, have a canonical organization such that every cortical region sends output from both layer 6 (L6) and layer 5 (L5) to the thalamus. Here we demonstrate a qualitative, region-specific difference
Martín Macías et al.
Frontiers in human neuroscience, 16, 1043501-1043501 (2022-12-13)
The ability to learn motor skills implicates an improvement in accuracy, speed and consistency of movements. Motor control is related to movement execution and involves corticospinal neurons (CSp), which are broadly distributed in layer 5B of the motor and somatosensory
Patrick Lüningschrör et al.
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