07-2101-M

Anti-phospho PIX Antibody (Ser340)

from rabbit, purified by affinity chromatography

• Synonym(s): Rho guanine nucleotide exchange factor (GEF) 7, PAK-interacting exchange factor beta, guanine nucleotide exchange factor 7, rho guanine nucleotide exchange factor 7, SH3 domain-containing proline-rich protein, p85, PAK3, Beta-Pix, P85COOL1, COOL-1, P85,
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology), human (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_001106989
• UniProt accession no.: Q9ES28
• target post-translational modification: phosphorylation (pSer340)
• Gene Information: human ... PAK3(5063)

Description

General description

p-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.

Specificity

This antibody recognizes PIX phosphorylated at Ser340.

Immunogen

Epitope: Phosphorylated Ser340

KLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.

Application

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

Quality

Evaluated by Western Blot in PIX transfected cell lysates.

Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.

Target description

~ 75 kDa

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.

Analysis Note

Control
PIX transfected cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1