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Key Documents

06-1049

Sigma-Aldrich

Anti-Axin-1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Axin-1, Axis inhibition protein 1, hAxin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_003493

UniProt accession no.

O15169

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Axin1(12005)

General description

Axin-1 is a beta-catenin destruction complex scaffold protein and a transcription factor of the Wnt signaling pathway. Variation in the Wnt signaling pathway has been associated with the formation of some primary human cancers. Axin-1 mutation has been linked to oral squamous cell carcinoma. Axin-1 binds to GSK3alpha and activates the Wnt signaling pathway which is shown to be associated with growth and development of cells. Axin-1 has also been implicated in Alzheimer′s disease.

Immunogen

KLH-conjugated linear peptide corresponding to human Axin-1.

Application

Anti-Axin-1 Antibody is an antibody against Axin-1 for use in WB, IC, IH.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Axin-1 in A431 and HeLa cells.

Immunohistochemistry Analysis: 1:1,000 dilution from a representative lot detected Axin-1 in human kidney and human skin tissues.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 on 10 µg of HEK293 cell lysate.

Target description

~110 kDa observed. An uncharacterized band appears at ~40 and 45 kDa in some lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Genetic evidence has indicated that β-catenin plays a vital role in glucose and lipid metabolism. Here, we investigated whether pyrvinium, an anthelmintic agent previously reported as a down-regulator of cellular β-catenin levels, conferred any metabolic advantages in treatment of metabolic
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