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Key Documents

05-1579

Sigma-Aldrich

Anti-Axin-1 Antibody, clone A5

clone A5, from mouse

Synonym(s):

Axis inhibition protein 1, axin 1, axis inhibitor 1, fused, mouse, homolog of

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

A5, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_033863.2

UniProt accession no.

O35625

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AXIN1(8312)
mouse ... Axin1(12005)

General description

Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.

Specificity

This antibody recognizes the N-terminus of Axin-1.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.

Application

Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.

Quality

Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.

Target description

~ 97 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chuifeng Fan et al.
Molecular carcinogenesis, 54 Suppl 1, E138-E147 (2014-09-30)
Our previous studies indicate that abnormal expression of several Wnt signaling molecules including Axin, Dvl and β-catenin are involved in proliferation, invasion and metastasis of lung cancer. Zbed3 was found to inhibit function of Axin-GSK3β complex and thus lead to
Phosphatidylinositol 3-kinase signaling does not activate the wnt cascade.
Ng, SS; Mahmoudi, T; Danenberg, E; Bejaoui, I; de Lau, W; Korswagen, HC; Schutte, M; Clevers, H
The Journal of Biological Chemistry null
Diego Ploper et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(5), E420-E429 (2015-01-22)
Canonical Wnt signaling plays an important role in development and disease, regulating transcription of target genes and stabilizing many proteins phosphorylated by glycogen synthase kinase 3 (GSK3). We observed that the MiT family of transcription factors, which includes the melanoma

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