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Key Documents

04-147

Sigma-Aldrich

Anti-ASC Antibody, clone 2EI-7

clone 2EI-7, from mouse

Synonym(s):

Caspase recruitment domain-containing protein 5, PYD and CARD domain containing, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain prot

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2EI-7, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_145182

UniProt accession no.

Q9ULZ3

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PYCARD(29108)

General description

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Irregular expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

Specificity

This antibody recognizes ASC.

Immunogen

Epitope: Unknown
Full length recombinant human ASC.

Application

Detect ASC using this Anti-ASC Antibody, clone 2EI-7 validated for use in WB, FC.
Research Category
Inflammation & Immunology
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot on mouse spleen tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ASC in mouse spleen tissue lysate.

Target description

~22 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse spleen tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tiantian Tang et al.
Nature communications, 8(1), 202-202 (2017-08-06)
The NLRP3 inflammasome can sense different pathogens or danger signals, and has been reported to be involved in the development of many human diseases. Potassium efflux and mitochondrial damage are both reported to mediate NLRP3 inflammasome activation, but the underlying
Andrea Borrego et al.
Frontiers in immunology, 13, 899569-899569 (2022-07-09)
We identified Pycard and BC017158 genes as putative effectors of the Quantitative Trait locus (QTL) that we mapped at distal chromosome 7 named Irm1 for Inflammatory response modulator 1, controlling acute inflammatory response (AIR) and the production of IL-1β, dependent
Sung-Soo Jung et al.
American journal of physiology. Lung cellular and molecular physiology, 308(10), L1058-L1067 (2015-03-15)
Inflammasomes are cytosolic protein complexes that promote the cleavage of caspase-1, which leads to the maturation and secretion of proinflammatory cytokines, including interleukin-1β (IL-1β) and IL-18. Among the known inflammasomes, the nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3)-dependent inflammasome is
Chromatin dynamics during DSB repair.
Martin Falk,Emilie Lukasova,Barbora Gabrielova,Vladan Ondrej,Stanislav Kozubek
Biochimica et Biophysica Acta null
The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization.
Ippagunta, SK; Malireddi, RK; Shaw, PJ; Neale, GA; Vande Walle, L; Green, DR; Fukui et al.
Nature Immunology null
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