Stability and Reproducibility of Zenix^® 300 under High Salt and High pH Mobile Phase Conditions

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777018)

mobile phase

50 mM sodium phosphate + 1 M NaCl, pH 8.5

flow rate

1.0 mL/min

pressure

1450 psi (100 bar)

column temp.

25 °C

detector

UV, 214 nm

injection

5 μL

sample

each compound 1 mg/mL except uracil, 0.1 mg/mL