HPLC Separation of an Oligonucleotide and Impurities on Proteomix^® SAX-NP5 versus Porous SAX

column

Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)

mobile phase

(A) 0.025 M Tris buffer pH9; (B) A + 1.0 M LiCi

gradient

30 to 75% B in 45 min

flow rate

1.0 mL/min

detector

UV, 260 nm

injection

50 μL

sample

50-mer Oligo