HPLC Analysis of N-Desmethylnefopam Enantiomers on Astec^® CHIROBIOTIC^® V2

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

mobile phase

[A] methanol; [B] acetic acid; [C] ammonium hydroxide; (100:0.1:0.02, A:B:C)

flow rate

1.2 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

5 μL

sample

2 mg/mL in methanol