HPLC Analysis of Methionine Enantiomers on Astec® CHIROBIOTIC® T2
CONDITIONS
column
CHIROBIOTIC T2, 25 cm x 4.6 mm I.D., 5 μm particles (16024AST)
mobile phase
[A] water; [B] acetonitrile; (25:75, A:B)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 220 nm
injection
1 μL
sample
1 mg/mL in methanol
Descrição
Informações legais
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC