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HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD) application for HPLC

Materiais

Padrão

Nº do produto
Descrição
Preços

T0625

Taurine

≥99%

56396

Cafeína

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

coluna analítica

Nº do produto
Descrição
Preços

53967-U

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

CONDITIONS

column

Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

1000 μg/mL in 10:90, water: acetonitrile

Descrição

Nota de análise

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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