HPLC Analysis of Angiotensins on Discovery^® BIO Wide Pore C18

column

Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)

mobile phase

[A] (10 mM NH₄OAc, pH 7):(50% CH₃CN in 20 mM NH₄OAc, pH 7), 65:35 ; [B] (10 mM NH₄OAc, pH 7):(50% CH₃CN in 20 mM NH₄OAc, pH 7), 25:75

gradient

0-100% B in 12.5 min

flow rate

1 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

6 μL (10 μg each) in water