HPLC Analysis of 2-AMP and Piroxicam on Ascentis® Express F5
CONDITIONS
column
Ascentis Express F5, 10 cm x 4.6 mm I.D., 2.7 μm particles (53590-U)
mobile phase
[A] 10 mM ammonium formate, pH=3; [B] acetonitrile; (75:25, A:B)
flow rate
0.8 mL/min
pressure
1635 psi (113 bar)
column temp.
35 °C
detector
UV, 230 nm
injection
5 μL
sample
100 μg/mL Piroxicam and 10 μg/mL 2-AMP in mobile phase
Descrição
Informações legais
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany