GC Analysis of cis/trans C18:1, C18:2, and C18:3 FAME Isomers on SP^®-2560

column

SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056) (24056)

oven

175 °C

inj. temp.

210 °C

detector

FID, 250 °C

carrier gas

helium, 20 cm/sec @ 175 °C

injection

1.0 μL, 100:1 split

sample

Mixture of C18:1, C18:2, and C18:3 FAMEs in methylene chloride