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Merck

Detection of canine visceral leishmaniasis by conjunctival swab PCR.

Revista da Sociedade Brasileira de Medicina Tropical (2016-05-11)
Vanessa Figueredo Pereira, Julia Cristina Benassi, Wilma Aparecida Starke-Buzetti, Diogo Tiago Silva, Helena Lage Ferreira, Lara Borges Keid, Rodrigo Martins Soares, Vera Letticie de Azevedo Ruiz, Trícia Maria Ferreira de Sousa Oliveira
RESUMO

Conjunctival swab PCR was evaluated as a tool to diagnose visceral leishmaniasis in dogs. Conjunctival swab PCR was compared to indirect immunofluorescence antibody test and blood PCR. Indirect immunofluorescence was significantly correlated with conjunctival swab PCR (p < 0.05), but not with blood PCR (p > 0.05). In addition, conjunctival swab PCR was significantly associated with presence of clinical symptoms (p < 0.05), whereas blood PCR was associated with absence of clinical symptoms (p < 0.05). Results indicate that conjunctival swab PCR is useful in epidemiological surveys of canine visceral leishmaniasis.

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Sigma-Aldrich
Anti-IgG canina (molécula inteira)–FITC, affinity isolated antibody, buffered aqueous solution