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Merck

Dynamic combinatorial mass spectrometry leads to metallo-beta-lactamase inhibitors.

Journal of medicinal chemistry (2008-01-22)
Benoît M R Liénard, Rebekka Hüting, Patricia Lassaux, Moreno Galleni, Jean-Marie Frère, Christopher J Schofield
RESUMO

The use of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial library of thiols/disulfides with the BcII metallo-beta-lactamase enabled the rapid identification of an inhibitor with a K(i) of < 1 microM. The study exemplifies the utility of protein-MS for screening dynamic mixtures of potential enzyme-inhibitors.

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Sigma-Aldrich
3-Mercaptopropionic acid, ≥99%
Sigma-Aldrich
3-Mercaptopropionic acid, ≥99.0% (HPLC)