ZRB1079

Anti-CNTNAP2 Antibody, clone 2D17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 2D17, recombinant monoclonal; monoclonal
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• peso molecular: calculated mol wt ~148.17 kDa; observed mol wt ~165 kDa
• reatividade de espécies: rat, human
• reatividade da espécie (prevista por homologia): mouse, monkey
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão UniProt: Q9UHC6
• categoria alternativa mais ecológica: Aligned,
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone 2D17 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Contactin-associated protein-like 2 (CNTNAP2). It targets an epitope within 17 amino acids from the C-terminal, cytoplasmic domain.

Imunogênio

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Contactin-associated protein-like 2 (CNTNAP2).

Aplicação

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CNTNAP2 in Rat brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CNTNAP2 in Human brain tissue lysate.

Affinity Binding Assay:: A representative lot of this antibody bound CNTNAP2 peptide with a KD of 3.7 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CNTNAP2 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CNTNAP2, clone 2D17 ZooMAb^®, Cat. No. ZRB1079, is a rabbit recombinant monoclonal antibody that specifically targets CNTNAP2 and is tested for use in Affinity Binding Assay, Immunohistochemistry, and Western Blotting.

Descrição-alvo

Contactin-associated protein-like 2 (UniProt: Q9UHC6; also known as Cell recognition molecule Caspr2, CNTNAP2) is encoded by the CNTNAP2 (also known as CASPR2, KIAA0868) gene (Gene ID: 26047) in human. CNTNAP2 is a single-pass type I membrane glycoprotein protein of the neurexin family that is predominantly expressed in the nervous system. It mediates synaptic cell adhesion and is required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. It also plays a role in the formation of functional distinct domains that are critical for saltatory conduction of nerve impulses in myelinated nerve fibers. CNTNAP2 is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 28-1262), a transmembrane domain (aa 1263-1283), and a cytoplasmic domain (aa 1284-1331). Knockdown of CNTNAP2 in cortical cultures is shown to impair the development of spines. Mutations in CNTNAP2 gene are known to be associated with intellectual disability, epilepsy, schizophrenia, language disorders, and autism. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gdalyahu A, et al., (2015). PLoS ONE. 10(5); e0125633).

forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstituição

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable