Z0627

Monoclonal Anti-ZAP-70 antibody produced in mouse

~1.5 mg/mL, clone 1E7.2, purified immunoglobulin, buffered aqueous solution

• Número MDL: MFCD00803577
• Código UNSPSC: 12352203
• NACRES: NA.44

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 1E7.2, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~70 kDa
• reatividade de espécies: human, mouse
• embalagem: antibody small pack of 25 μL
• concentração: ~1.5 mg/mL
• técnica(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1.0 μg/mL using Jurkat cell extract
• Isotipo: IgG1
• nº de adesão UniProt: P43403
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ZAP70(7535) ; mouse ... Zap70(22637)

Descrição

Descrição geral

Monoclonal Anti-ZAP-70 (mouse IgG1 isotype) is derived from the hybridoma 1E7.2 produced by the fusion of mouse myeloma cells and splenocytes from mice. ZAP-70 is a protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells.

Imunogênio

The immunogen sequence is conjugated to KLH.

peptide corresponding to amino acids 282-307 of human ZAP-70.

Aplicação

Monoclonal Anti-ZAP-70 antibody has been used in

• enzyme linked immunosorbent assay (ELISA)
• immunoblottin
• flow cytometry
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-ZAP-70 antibody is suitable for use in western blot (0.5-1.0 μg/mL using Jurkat cell extract), flow cytometry, immunocytochemistry, immunoprecipitation, microarray and indirect ELISA.

Ações bioquímicas/fisiológicas

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the zeta chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling . Lack of ZAP-70 leads to an absence of antigen-specific response and also causes impaired T cell differentiation . Anti-ZAP-70 antibody is specific for human and mouse ZAP-70 (approx. 70 kDa).

Zeta-chain-associated protein kinase70 (ZAP-70) is required for T-cell activation and is activated by(Tyrosine protein kinase) Lck-mediated phosphorylation of its tyrosine residues. Mutation of the gene that creates a defective ZAP-70 results in severe combined immunodeficiency syndrome (SCID).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)