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Key Documents

WH0025813M4

Sigma-Aldrich

Monoclonal Anti-SAMM50 antibody produced in mouse

clone 2A9, purified immunoglobulin, buffered aqueous solution

Sinônimo(s):

Anti-CGI51, Anti-OMP85, Anti-SAM50, Anti-TOB55, Anti-TRG3, Anti-YNL026W, Anti-sorting and assembly machinery component 50 homolog (S. cerevisiae)

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

2A9, monoclonal

forma

buffered aqueous solution

reatividade de espécies

mouse

técnica(s)

indirect ELISA: suitable
western blot: 1-5 μg/mL

Isotipo

IgG2bκ

nº de adesão GenBank

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... SAMM50(25813)

Descrição geral

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAM complex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005 [PubMed 15644312]).[supplied by OMIM

Imunogênio

SAMM50 (AAH07830, 1 a.a. ~ 468 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLKVNQELAGYTGGDVSFIKEDFELQLNKQLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSMHSIGPQSEGDYLGGEAYWAGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAECIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQFGAGIRFL

Ações bioquímicas/fisiológicas

Sorting and assembly machinery (SAM) 50 helps in aggregation of β-barrel proteins into the outer mitochondrial membrane (OMM). SAM 50, along with mitofilin and coiled-coil-helix-coiled-coil-helix domain containing 3 (CHCHD3), plays a vital role in maintaining mitochondrial shape, the morphology of cristae, and the assembly of respiratory complexes. Elevated expression of SAMM50 leads to mitochondrial fragmentation in HeLa cells under the influence of dynamin-related protein (Drp) 1. Depleted expression of SAM 50 affects assembly and steady-state level of voltage-dependent anion-selective channel (VDAC), which is essential for transport of metabolite across OMM.

forma física

Solution in phosphate buffered saline, pH 7.4

Informações legais

GenBank is a registered trademark of United States Department of Health and Human Services

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análise (COA)

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Shuo Liu et al.
FEBS letters, 590(9), 1313-1323 (2016-04-10)
Mitochondrial fission and fusion activities are important for cell survival and function. Drp1 is a GTPase protein responsible for mitochondrial division, and SAMM50 is responsible for protein sorting and assembly. We demonstrated that SAMM50 overexpression results in Drp1-dependent mitochondrial fragmentation
Vera Kozjak et al.
The Journal of biological chemistry, 278(49), 48520-48523 (2003-10-23)
The preprotein translocase of the outer mitochondrial membrane (TOM complex) contains one essential subunit, the channel Tom40. The assembly pathway of the precursor of Tom40 involves the TOM complex and the sorting and assembly machinery (SAM complex) with the non-essential
Vera Kozjak-Pavlovic et al.
EMBO reports, 8(6), 576-582 (2007-05-19)
Voltage-dependent anion-selective channel (VDAC) is a beta-barrel protein in the outer mitochondrial membrane that is necessary for metabolite exchange with the cytosol and is proposed to be involved in certain forms of apoptosis. We studied the biogenesis of VDAC in
Christine Ott et al.
Molecular and cellular biology, 32(6), 1173-1188 (2012-01-19)
Mitochondria possess an outer membrane (OMM) and an inner membrane (IMM), which folds into invaginations called cristae. Lipid composition, membrane potential, and proteins in the IMM influence organization of cristae. Here we show an essential role of the OMM protein

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