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Key Documents

SRP8023

Sigma-Aldrich

PARG human

recombinant, expressed in Sf21 cells, His tagged, >95% (SDS-PAGE)

Sinônimo(s):

FNDC5 (cleaved), Poly(ADP-ribose) glycohydrolase

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About This Item

Número CAS:
9068-16-0
Código UNSPSC:
12352200
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in Sf21 cells

etiqueta

His tagged

Ensaio

>95% (SDS-PAGE)

forma

liquid

peso molecular

~110 kDa by SDS-PAGE

embalagem

pkg of 2 μg

concentração

≥0.2 mg/mL

técnica(s)

cell based assay: suitable

cor

clear

solubilidade

water: soluble

nº de adesão UniProt

Q86W56

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... PARG(8505)

Descrição geral

Research area: Cell signaling

Aplicação

PARG human has been used in the digestion of sciatic nerve sections, in co-immunoprecipitation, in in vitro glycohydrolase activity assay

Ações bioquímicas/fisiológicas

Poly(ADP-ribose) glycohydrolase(PARG) hydrolyzes poly(ADP-ribose) at glycosidic(1′′-2′′) linkage of ribose-ribose bond to produce free ADP-ribose. It is an endo- and exo-glycosidase. PARG plays arole in DNA repair and replication. Inhibited or depleted PARG in cells aremore prone to DNA-damaging agents. PARG also averts the accumulation of PAR inthe cytoplasm and parthanatos, a caspase-independent PAR-mediated type of celldeath.

forma física

Solution in 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2% NP-40, 50 mM imidazole and 10% glycerol.

Outras notas

Human PARG is fused to a His-tag.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H360D

Classificações de perigo

Repr. 1B

Código de classe de armazenamento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Sara C Larsen et al.
Cell reports, 24(9), 2493-2505 (2018-08-30)
ADP-ribosylation (ADPr) is a reversible posttranslational modification involved in a range of cellular processes. Here, we report system-wide identification of serine ADPr in human cells upon oxidative stress. High-resolution mass spectrometry and unrestricted data processing confirm that serine residues are
Jeannette Abplanalp et al.
Nature communications, 8(1), 2055-2055 (2017-12-14)
ADP-ribosylation is a posttranslational modification that exists in monomeric and polymeric forms. Whereas the writers (e.g. ARTD1/PARP1) and erasers (e.g. PARG, ARH3) of poly-ADP-ribosylation (PARylation) are relatively well described, the enzymes involved in mono-ADP-ribosylation (MARylation) have been less well investigated.
Sara C Buch-Larsen et al.
Cell reports, 32(12), 108176-108176 (2020-09-24)
ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide range of cellular processes. Mass spectrometry (MS)-based analysis of ADPr under physiological conditions, without relying on genetic or chemical perturbation, has been hindered by technical limitations. Here
Xiao-Nan Zhang et al.
Nature communications, 10(1), 4196-4196 (2019-09-15)
Nicotinamide adenine dinucleotide (NAD+)-dependent ADP-ribosylation plays important roles in physiology and pathophysiology. It has been challenging to study this key type of enzymatic post-translational modification in particular for protein poly-ADP-ribosylation (PARylation). Here we explore chemical and chemoenzymatic synthesis of NAD+
Michelle Schacke et al.
International journal of molecular sciences, 20(3) (2019-01-30)
Poly- adenosine diphosphate (ADP)-ribose (PAR) is a polymer synthesized as a posttranslational modification by some poly (ADP-ribose) polymerases (PARPs), namely PARP-1, PARP-2, tankyrase-1, and tankyrase-2 (TNKS-1/2). PARP-1 is nuclear and has also been detected in extracellular vesicles. PARP-2 and TNKS-1/2

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