SRP6546

Procathepsin L human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinônimo(s): CTSL, CTSL1
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥90% (SDS-PAGE)
• forma: liquid
• peso molecular: 39.4 kDa (18-333 aa + N-terminal poly-his tag.)
• embalagem: pkg of 1 mg; pkg of 100 μg
• concentração: 1 mg/mL
• nº de adesão UniProt: P07711
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... CTSL1(1514)

Descrição

Descrição geral

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

forma física

1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable