SRP5132

SMAD3, GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinônimo(s): DKFZP586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396
• Número CAS: 184379-52-0
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥70% (SDS-PAGE)
• forma: buffered aqueous glycerol solution
• peso molecular: ~77 kDa
• nº de adesão NCBI: NM_005902
• aplicação(ões): cell analysis
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... SMAD3(4088)

Descrição

Descrição geral

SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor. SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFβ response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.

forma física

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Nota de preparo

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable