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Merck
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Key Documents

SRP3070

Sigma-Aldrich

IGF-II human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinônimo(s):

Insulin-like Growth Factor-II

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥98% (HPLC)
≥98% (SDS-PAGE)

forma

lyophilized

potência

≤2.0 ng/mL ED50

peso molecular

7.5 kDa

embalagem

pkg of 50 μg

técnica(s)

cell culture | mammalian: suitable

Impurezas

<0.1 EU/μg endotoxin, tested

cor

off-white to yellow

nº de adesão UniProt

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... IGF2(3481)

Descrição geral

Insulin-like growth factors (IGFs) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.

Aplicação

IL-4 from mouse has been used to activate B-cells.

Ações bioquímicas/fisiológicas

The insulin-like growth factors (IGFs) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro.

Sequência

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

forma física

Lyophilized with no additives.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

Role of IGF-I Signaling in Muscle Bone Interactions
Daniel D, et.al
Bone, 80, 79-88 (2015)
Type 1 Insulin-like Growth Factor Receptor (IGF-IR) Signaling Inhibits Apoptosis Signal-regulating Kinase 1 (ASK1)*
Veronica Galvan
The Journal of Biological Chemistry, 13325-13332 (2003)
Role of IGF-I Signaling in Muscle Bone Interactions
Daniel D Bikle
Bone, 80, 79-88 (2015)
Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation
Kyong-RimKieffer-Kwon
Molecular Cell, 67(4), 566-578 (2017)
A J D'Ercole
Endocrinology and metabolism clinics of North America, 25(3), 573-590 (1996-09-01)
The IGFs are essential stimulators of fetal and postnatal growth. Their signals are mediated predominantly by the type I IGF receptor, but another as yet unidentified receptor seems to mediate some actions, especially some of those for IGF-II. During organogenesis

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